古志遠(yuǎn)教授課題組和David Chen教授課題組合作在ANALYTICA CHIMICA ACTA發(fā)表研究論文
A hydrophilic two-dimensional titanium-based metal-organic framework nanosheets for
specific enrichment of glycopeptides
Yang, SS (Yang, Shi-Shu)[ 1 ] ; Wang, C (Wang, Chen)[ 1 ] ; Yu, XZ (Yu, Xizhong)[ 2 ] ;
Shang, WB (Shang, Wenbin)[ 2 ] ; Chen, DDY (Chen, David Da Yong)[ 1,3 ]*; Gu, ZY (Gu, Zhi-
Yuan)[ 1 ]*（古志遠(yuǎn)）
[ 1 ]Nanjing Normal Univ, Jiangsu Key Lab Biofunct Mat, Jiangsu Collaborat Innovat Ctr
Biomed Funct Mat, Coll Chem & Mat Sci, Nanjing 210023, Peoples R China
[ 2 ]Nanjing Univ Chinese Med, Key Lab Metab Dis Chinese Med, Coll Clin Med 1, Nanjing
210023, Peoples R China
[ 3 ]Univ British Columbia, Dept Chem, Vancouver, BC V6T 1Z1, Canada
ANALYTICA CHIMICA ACTA，202007,1119,60-67
A hydrophilic two-dimensional titanium-based metal-organic framework (MOF) nanosheets were
prepared and applied to improve the glycopeptides enrichment efficiency. Seventeen N-linked
glycopeptides from horseradish peroxidase (HRP) tryptic digests were readily identified and
the results showed highly improved signal intensities. As the molar ratio of the mixture of
tryptic digests of HRP, glycoprotein (HRP), and nonglycoprotein (BSA) reached 1:800:800,
good selectivity of the Ti-MOF nanosheets were still clearly observed. As to human serum
samples, 41 glycoproteins and 66 glycopeptides corresponding to 68 N-glycosylation sites
were finally detected, which showed the good ability of the nanosheets material to enrich
N-linked glycopeptides from complex human serum samples. Moreover, the Ti-MOF nanosheets
exhibited unique stability and reusability even after being regenerated and reused for five
times. It also performed a high sensitivity (1 x 10(-10) M HRP), a good enrichment capacity
(250 mg/g) for glycopeptides enrichment and a high enrichment recovery of 78%, suggesting a
great application prospect for the enrichment of N-linked glycopeptides and the analysis
glycoproteomic research.
文章鏈接：
https://www.sciencedirect.com/science/article/pii/S0003267020304839?via%3Dihub
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