戴志暉教授課題組在ANALYTICA CHIMICA ACTA發(fā)表研究論文
Bimetallic metal-organic framework for enzyme immobilization by biomimetic mineralization: Constructing a mimic enzyme and simultaneously immobilizing natural enzymes
Wang, JN (Wang, Junning); Bao, MY (Bao, Mengyuan); Wei, TX (Wei, Tianxiang); Wang, ZY (Wang, Zhaoyin); Dai, ZH (Dai, Zhihui)[ 1 ]*（戴志暉）
[ 1 ] Nanjing Normal Univ, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Sch Chem & Mat Sci, Nanjing 210023, Peoples R China
[ 2 ] Nanjing Normal Univ, Jiangsu Key Lab Biofunct Mat, Sch Chem & Mat Sci, Nanjing 210023, Peoples R China
ANALYTICA CHIMICA ACTA，202002,1098,148-154
An iron-nickel bimetallic metal-organic framework (FeNi-MOF) with inner peroxidase-like activity as a support for enzyme immobilization was synthesized for the first time. And the GOx-mediated synthesis of mimic multienzyme system (GOx/FeNi-MOF) was obtained by a one-step biomimetic mineralization. FeNi-MOF played a dual role of a peroxidase mimic and a protective coating. Therefore, the obtained multienzyme system showed both peroxidase-like activity of FeNi-MOF and the biological activity of natural enzyme. FeNi-MOF acted as a peroxidase mimic, showing a higher affinity for hydrogen peroxide (H2O2). The enhanced catalytic activity of the FeNi-MOF may result from synergistic effect between iron and nickel. In addition, the GOx/FeNi-MOF was selected as a proof of concept and succeeded in a one-step colorimetric detection of glucose by tandem catalysis. A small amount of the product was used for glucose detection, and the detection range was 0.3-35 mM, which indicated that our sensor can meet the clinical needs for diagnose diabetes.
文章鏈接：
https://www.sciencedirect.com/science/article/pii/S0003267019313960?via%3Dihub
版權(quán)與免責(zé)聲明：本網(wǎng)頁(yè)的內(nèi)容由收集互聯(lián)網(wǎng)上公開(kāi)發(fā)布的信息整理獲得。目的在于傳遞信息及分享，并不意味著贊同其觀點(diǎn)或證實(shí)其真實(shí)性，也不構(gòu)成其他建議。僅提供交流平臺(tái)，不為其版權(quán)負(fù)責(zé)。如涉及侵權(quán)，請(qǐng)聯(lián)系我們及時(shí)修改或刪除。郵箱：sales@allpeptide.com