久久精品酒店无码视频_g∨天堂在线观看免费_av之高清在线_午夜福利资源片在线

Li, MM (Li, Manman)[ 1 ] ; Sun, YS (Sun, Yusheng)[ 1 ] ; Ma, CJ (Ma, Chenjun)[ 1 ] ; Hua, YY (Hua, Yingying)[ 1 ] ; Zhang, LF (Zhang, Liefeng)[ 1 ] *; Shen, J (Shen, Jian)[ 2 ]*（沈?。?/p>

[ 1 ]? Nanjing Normal Univ, Sch Food Sci & Pharmaceut Engn, Nanjing 210046, Peoples R China
[ 2 ]? Nanjing Normal Univ, Coll Chem & Mat Sci, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Jiangsu Key Lab Biomed Mat, Nanjing 210046, Peoples R China

JOURNAL OF PHARMACEUTICAL SCIENCES 2021,110,268-279

The aim of the study is to design octaarginine (R8)-modified insulin-alginate nanoparticles (INS-SA/R8 NPs) as the oral insulin delivery system, and further investigate its penetrating mechanism. The characterization results indicated that the surface of INS-SA/R8 NPs was smooth and the average diameter was about 300 nm. INS-SA/R8 NPs exhibited a stronger stability in the simulated gastrointestinal fluids and had a better controlled release than unmodified alginate nanoparticles (INS-SA NPs). Moreover, INSSA/R8 NPs group had the strongest insulin transport capacity and the largest amount of insulin uptake in all experimental groups. Most importantly, the improvement of insulin intestinal uptake was further confirmed in rat intestine in vivo, and its penetrating mechanism might be involved in the production of endogenous nitric oxide (NO) signal molecule. In addition, in vivo hypoglycemic studies showed that orally administrated INS-SA/R8 NPs produced a better hypoglycemic effect as compared with INS-SA NPs in diabetic rats. Meanwhile, from the cytotoxicity analysis, INS-SA/R8 NPs were safe for oral administration. Taken together, INS-SA/R8 NPs was a good oral insulin delivery system, which might also be suitable for other protein drugs. (C) 2020 American Pharmacists Association (R). Published by Elsevier Inc. All rights reserved.