作 者：王露、劉文靜、趙衛(wèi)東、宋根娣、王貴栓、王小蓉、孫斐
Abstract：
In mammalian meiosis, the X and Y chromosomes are largely unsynapsed and transcriptionally silenced during the pachytene stage of meiotic prophase (meiotic sex chromosome inactivation), forming a specialized nuclear territory called sex or XY body. An increasing number of proteins and non-coding RNAs were found to localize to the sex body and take part in influencing expression of sex chromosome genes. Cdk2 (cyclin-dependent kinase 2)-/- spermatocytes show incomplete sex chromosome pairing. Here, we further show that p-CDK239 (phosphorylation of CDK2 isoform 1 (39 kD) on threonine 160) localizes to the sites of asynapsis and the sex body, interacting with phosphorylated H2AX (gamma-H2AX). Meanwhile, p-CDK239 is frequently mislocalized throughout the sex body and meiotic sex chromosome inactivation is disrupted in PWK×C57BL/6J hybrid mice. Furthermore, pachytene spermatocytes treated with mevastatin (an inhibitor of p-CDK2) showed overexpression of sex chromosome-linked genes. Our results highlight an important role for p-CDK239 in influencing silencing of the sex chromosomes during male meiosis by interacting with gamma-H2AX.?
?
DOI: 10.1095/biolreprod.113.116624
?
版權(quán)與免責(zé)聲明：本網(wǎng)頁的內(nèi)容由收集互聯(lián)網(wǎng)上公開發(fā)布的信息整理獲得。目的在于傳遞信息及分享，并不意味著贊同其觀點(diǎn)或證實(shí)其真實(shí)性，也不構(gòu)成其他建議。僅提供交流平臺，不為其版權(quán)負(fù)責(zé)。如涉及侵權(quán)，請聯(lián)系我們及時(shí)修改或刪除。郵箱：sales@allpeptide.com