400-998-5282
專注多肽 服務科研
400-998-5282
專注多肽 服務科研
編號：155030
CAS號：
單字母：H2N-ELLRESLAAELRG-C(Acm)-GPRFGKHLLSYCPMPEKTFTTTPGGWL-OH
多肽H2N-Glu-Leu-Leu-Arg-Glu-Ser-Leu-Ala-Ala-Glu-Leu-Arg-Gly-Cys(Acm)-Gly-Pro-Arg-Phe-Gly-Lys-His-Leu-Leu-Ser-Tyr-Cys-Pro-Met-Pro-Glu-Lys-Thr-Phe-Thr-Thr-Thr-Pro-Gly-Gly-Trp-Leu-COOH的合成步驟：
1、合成CTC樹脂：稱取1.27g CTC Resin（如初始取代度約為0.74mmol/g）和1.13mmol Fmoc-Leu-OH于反應器中，加入適量DCM溶解氨基酸（需要注意，此時CTC樹脂體積會增大好幾倍，避免DCM溶液過少），再加入2.82mmol DIPEA（Mw：129.1,d：0.740g/ml），反應2-3小時后，可不抽濾溶液，直接加入1ml的HPLC級甲醇，封端半小時。依次用DMF洗滌2次，甲醇洗滌1次，DCM洗滌一次，甲醇洗滌一次，DCM洗滌一次，DMF洗滌2次（這里使用甲醇和DCM交替洗滌，是為了更好地去除其他溶質，有利于后續(xù)反應）。得到 Fmoc-Leu-CTC Resin。結構圖如下：
2、脫Fmoc：加3倍樹脂體積的20%Pip/DMF溶液，鼓氮氣30分鐘，然后2倍樹脂體積的DMF 洗滌5次。得到 H2N-Leu-CTC Resin 。（此步驟脫除Fmoc基團，茚三酮檢測為藍色，Pip為哌啶）。結構圖如下：
3、縮合：取2.82mmol Fmoc-Trp(Boc)-OH 氨基酸，加入到上述樹脂里，加適當DMF溶解氨基酸，再依次加入5.64mmol DIPEA，2.68mmol HBTU。反應30分鐘后，取小樣洗滌，茚三酮檢測為無色。用2倍樹脂體積的DMF 洗滌3次樹脂。（洗滌樹脂，去掉殘留溶劑，為下一步反應做準備）。得到Fmoc-Trp(Boc)-Leu-CTC Resin。氨基酸：DIPEA：HBTU：樹脂=3：6：2.85：1（摩爾比）。結構圖如下：
4、依次循環(huán)步驟二、步驟三，依次得到
H2N-Trp(Boc)-Leu-CTC Resin
Fmoc-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Ala-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Ala-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Ala-Ala-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Ala-Ala-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Leu-Ala-Ala-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Leu-Ala-Ala-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Ser(tBu)-Leu-Ala-Ala-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Ser(tBu)-Leu-Ala-Ala-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Glu(OtBu)-Ser(tBu)-Leu-Ala-Ala-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Glu(OtBu)-Ser(tBu)-Leu-Ala-Ala-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Arg(Pbf)-Glu(OtBu)-Ser(tBu)-Leu-Ala-Ala-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Arg(Pbf)-Glu(OtBu)-Ser(tBu)-Leu-Ala-Ala-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Leu-Arg(Pbf)-Glu(OtBu)-Ser(tBu)-Leu-Ala-Ala-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Leu-Arg(Pbf)-Glu(OtBu)-Ser(tBu)-Leu-Ala-Ala-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Ser(tBu)-Leu-Ala-Ala-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
H2N-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Ser(tBu)-Leu-Ala-Ala-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
Fmoc-Glu(OtBu)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Ser(tBu)-Leu-Ala-Ala-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin
以上中間結構，均可在專肽生物多肽計算器-多肽結構計算器中，一鍵畫出。
最后再經過步驟二得到 H2N-Glu(OtBu)-Leu-Leu-Arg(Pbf)-Glu(OtBu)-Ser(tBu)-Leu-Ala-Ala-Glu(OtBu)-Leu-Arg(Pbf)-Gly-Cys(Acm)-Gly-Pro-Arg(Pbf)-Phe-Gly-Lys(Boc)-His(Trt)-Leu-Leu-Ser(tBu)-Tyr(tBu)-Cys(Trt)-Pro-Met-Pro-Glu(OtBu)-Lys(Boc)-Thr(tBu)-Phe-Thr(tBu)-Thr(tBu)-Thr(tBu)-Pro-Gly-Gly-Trp(Boc)-Leu-CTC Resin，結構如下：
5、切割：6倍樹脂體積的切割液（或每1g樹脂加8ml左右的切割液），搖床搖晃 2小時，過濾掉樹脂，用冰無水乙醚沉淀濾液，并用冰無水乙醚洗滌沉淀物3次，最后將沉淀物放真空干燥釜中，常溫干燥24小試，得到粗品H2N-Glu-Leu-Leu-Arg-Glu-Ser-Leu-Ala-Ala-Glu-Leu-Arg-Gly-Cys(Acm)-Gly-Pro-Arg-Phe-Gly-Lys-His-Leu-Leu-Ser-Tyr-Cys-Pro-Met-Pro-Glu-Lys-Thr-Phe-Thr-Thr-Thr-Pro-Gly-Gly-Trp-Leu-COOH。結構圖見產品結構圖。
切割液選擇：1）TFA：H2O=95%：5%
2）TFA：H2O：TIS=95%：2.5%：2.5%
3）三氟乙酸：茴香硫醚：1，2-乙二硫醇：苯酚：水=87.5%：5%：2.5%：2.5%：2.5%
（前兩種適合沒有容易氧化的氨基酸，例如Trp、Cys、Met。第三種適合幾乎所有的序列。）
6、純化凍干：使用液相色譜純化，收集目標峰液體，進行凍干，獲得蓬松的粉末狀固體多肽。不過這時要取小樣復測下純度 是否目標純度。
7、最后總結：
杭州專肽生物技術有限公司（ALLPEPTIDE http://amynixphotography.com）主營定制多肽合成業(yè)務，提供各類長肽，短肽，環(huán)肽，提供各類修飾肽，如：熒光標記修飾（CY3、CY5、CY5.5、CY7、FAM、FITC、Rhodamine B、TAMRA等），功能基團修飾肽（疊氮、炔基、DBCO、DOTA、NOTA等），同位素標記肽（N15、C13），訂書肽（Stapled Peptide）,脂肪酸修飾肽（Pal、Myr、Ste），磷酸化修飾肽（P-Ser、P-Thr、P-Tyr），環(huán)肽（酰胺鍵環(huán)肽、一對或者多對二硫鍵環(huán)），生物素標記肽，PEG修飾肽，甲基化修飾肽等。
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